High-quality and accurate ELISA assays can be used for drug discovery, disease diagnosis, and several other purposes in the field of medicine. However, performing a successful ELISA assay is no easy task, and the best option is to seek the help of experienced professionals. A reputed laboratory with years of experience in the domain of assays and testing will be able to implement the best practices and tips for conducting a successful and accurate ELISA Assay.
Consistency, reproducibility, sensitivity, and accuracy are some of the factors that should be kept in mind when an ELISA assay is being set up and performed. Some tips for performing successful assays include:
- Optimize the ELISA assay for sensitivity.
- Do not mix the components of different kit logs to maintain accuracy.
- Samples and test results must be reproducible across different assays.
- Low coefficient of variability between sample replicates should be maintained.
- Serial dilution is a must for reliable and reproducible sample curves.
- To maximize accuracy in sample analysis, dilution must be optimized.
- All suggested background wells should be included in the ELISA assay.
- Use the recommended buffers and diluents for sample preparation and standard curves.
- Incubating guidelines such as temperature, time, and shaking should be adhered to.
- Assay plates must be read at the correct wavelength for optimal data analysis.
By following these simple tips, researchers and scientists conducting ELISA assays can maximize their chances of success while minimizing the risk of an error which could invalidate the results of their test or assay.